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KMID : 0371320100780020111
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Journal of the Korean Surgical Society
2010 Volume.78 No. 2 p.111 ~ p.115
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Detection and Typing of Human Papillomavirus in Anal Condyloma Acuminatum of HIV-positive Patients
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Moon Suk-Bae
Moon Sang-Hui
Park Kyu-Joo
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Abstract
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Purpose: Anal condyloma is an epithelial proliferative lesion caused by human papillomavirus (HPV) infection. The present study analyzed the HPV types detected in HIV (＋) Korean anal condyloma using PCR-based DNA microarray.
Methods: DNA was extracted from the condyloma tissue of 17 patients including 9 HIV (＋) patients (M：F=15：2, mean age 35 years, 22∼59 years). The 1st PCR was performed with a general primer on L1 region, and nested PCR on the products of the 1st PCR. PCR products were hybridized with a DNA chip.
Results: Fourteen patients (9 HIV (＋), 5 HIV (?)) showed positive HPV DNA. Overall, type 6 was the most common (N=11), and type 11 (N=6), type 53 (N=3) in order. Among HIV (＋) patients, type 6 was also the most common (N=7), then type 11 (N=5) and type 53 (N=3). In contrast to the HIV (?) patients, 5 patients (55.6%) proved to have multiple infections in HIV (＋) patients (2 double, 2 triple, 1 quadruple infection). Four of 9 HIV (＋) patients (44.5%) showed co-infection with high-risk HPV.
Conclusion: Multiple infection and co-infection with high-risk types are more prevalent in HIV (＋) condyloma patients compared to HIV (?) patients. HPV types on HIV (＋) male anal condyloma are similar to those detected in the Korean female uterine cervix.
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KEYWORD
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Anal condyloma acuminatum, Human papillomavirus:Human immunodeficiency virus, Polymerase chain reaction, DNA microarray
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